SYNTHESIS OF BIOTINYLATED FERROCENE, AND ITS APPLICATION AS A REDOX PROBE FOR ELECTROCHEMICAL IMMUNOSENSOR

Le Huu Quynh Anh

Abstract


The coupling compound of Biotin (Vitamin H) and redox molecule ferrocene through
covalent bonding was studied. Synthesis and structure identification of Biotinylated 1 - (- 2-
aminoethyl) ferrocene were performed by 1H-NMR, 13C-NMR and MS. The ultimate goal of this
paper is to develop derivatives of ferroceneservingas an electrochemical probe that would
enhance the performance of immunosensor.An immunosensor based on polypyrrole-streptavidin
layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The
measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry
(DPV) method and underlined a variation of redox properties upon the antigen interaction. The
introduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a good
sensitivity of the biosensor with a detection limit of 0.16 pg.mL-1.


Keywords


immunosensor, ferrocene, redox probe, electrochemical detection, polypyrrole.

Full Text:

PDF


DOI: https://doi.org/10.15625/2525-2518/54/2C/11873 Display counter: Abstract : 76 views. PDF : 77 views.

Refbacks

  • There are currently no refbacks.


Index: Google Scholar; Crossref; VCGate; Asean Citation Index

Published by Vietnam Academy of Science and Technology