Antibacterial activity of Piper betle extracts on Helicobacter pylori and identification of potential compounds

Nguyen Thi Huyen Trang, Do Thi Thanh Trung, Nguyen Thi Thanh Thi, Pham Thi Luong Hang, Pham Thi Vinh Hoa, Pham Bao Yen

Abstract


Helicobacter pylori is one of the most common infectious bacteria in the world that causes gastric diseases leading to cancer. The increase of multiple antibiotic resistance rates of H. pylori have been reported worldwide. Thus, development of novel drugs is urgently required. Piper betle has many therapeutic values in traditional medicine. In this study, therefore, we investigated antibacterial activity of P. betle extracts and their fractions against a H. pylori strain isolated in Vietnam. The agar disk diffusion assay showed inhibition zone of ethyl acetate extract and methanol extract from P. betle leaf that of were 46 mm and 32 mm in diameter, respectively. After fractionation of the ethyl acetate extract through silica gel column chromatography, two peaks, PD2 and PD3, out of 12 fractions showed the strongest antibacterial activity. PD2 was sub-fractionated further by re-chromatography on the silica gel column, and subfraction TK12 gave best resolution on LC-MS analysis. Finally, 4 potential compounds, quercetrin, calodenin B, vitexin and plicatipyrone, were identified in TK12 fraction.

 


Keywords


Helicobacter pylori, medicinal plants, gastric disease, Piper betle, compound.

References


Agarwal A. K., Tripathi S. K., Xu T., Jacob M. R., Li X.-C. and Clark A. M., 2012. Exploring the molecular basis of antifungal synergies using genome-wide approaches. Frontiersin Microbiology, 3: 115.

Ali I., F. G. Khan, K. A. Suri, B. D. Gupta, N. K. Satti, P. Dutt, F. Afrin, G. N. Qazi and I. A. Khan, 2010. In vitro antifungal activity of hydroxychavicol isolated from Piper betle L. Annals of clinical microbiology and antimicrobials, 9(1): 7.

Arawwawala, L. D. A. M., Arambewela, L. S. R., & Ratnasooriya, W. D., 2014. Gastroprotective effect of Piper betle Linn. leaves grown in Sri Lanka. Journal of Ayurveda and integrative medicine, 5(1): 38.

Binh T. T., S. Shiota, L. T. Nguyen, D. D. Ho, H. H. Hoang, L. Ta, D. T. Trinh, T. Fujioka and Y. Yamaoka, 2013. The incidence of primary antibiotic resistance of Helicobacter pylori in Vietnam. Journal of clinical gastroenterology, 47(3): 233.

Chinou I. B., V. Roussis, D. Perdetzoglou, O. Tzakou and A. Loukis, 1997. Chemical and Antibacterial Studies of two Helichrysum Species of Greek Origin1. Planta medica, 63(02): 181–183.

Eusebi L. H., R. M. Zagari and F. Bazzoli, 2014. Epidemiology of Helicobacter pylori infection. Helicobacter, 19(s1): 1–5.

Fock K. M. and T. L. Ang, 2010. Epidemiology of Helicobacter pylori infection and gastric cancer in Asia. Journal of gastroenterology and hepatology, 25(3): 479–486.

Frenck Jr, R. W., & Clemens, J., 2003. Helicobacter in the developing world. Microbes and infection, 5(8): 705–713.

Ghosh R., K. Darin, P. Nath and P. Deb., 2014. An overview of various Piper species for their biological activities. International Journal of Pharmaceutical Sciences Review and Research, 3(1): 67–75.

Hang T. L. Pham, Lien T. T. Nguyen, Tuan A. Duong, Dung T. T Bui, Que T. Doan, Ha T. T. Nguyen, Sabine Mundt, 2017. Diversity and bioactivities of nostocacean cyanobacteria isolated from paddy soil in Vietnam. Systematic and Applied Microbiology, 40: 470–481.

Mitscher L. A., Leu R. P., Bathala M. S., Wu W. N., Beal J. L., 1972. Antimicrobial agents from higher plants-I: introduction, rationale and methodology. Lloydia, 35: 157–166.

Nguyen Van Toai, 2003. Initial study of anti- Helicobacter pylori by total extract from Piper betle in in vitro and pre-clinical trial, PhD Thesis.

Prabhu M., K. Platel, G. Saraswathi and K. Srinivasan, 1995. Effect of orally administered betel leaf (Piper betle Linn.) on digestive enzymes of pancreas and intestinal mucosa and on bile production in rats. Indian journal of experimental biology, 33(10): 752–756.

Pradhan D., K. Suri, D. Pradhan and P. Biswasroy, 2013. Golden Heart of the Nature: Piper betle L. Journal of Pharmacognosy and Phytochemistry, 1(6): 147–167.

Snow Setzer M., J. Sharifi-Rad and W. N. Setzer, 2016. The Search for Herbal Antibiotics: An In-Silico Investigation of Antibacterial Phytochemicals. Antibiotics, 5(3): 30.

Tang S., P. Bremner, A. Kortenkamp, C. Schlage, A. I. Gray, S. Gibbons and M. Heinrich, 2003. Biflavonoids with cytotoxic and antibacterial activity from Ochna macrocalyx. Planta medica, 69(03): 247–253.




DOI: https://doi.org/10.15625/0866-7160/v41n4.13876 Display counter: Abstract : 67 views.

 

                 

Editorial Office:

1st Floor, A16 Building, 18B Hoang Quoc Viet Street, Cau Giay District, Hanoi, Vietnam

Tel: (+84) 24 3791 7101

Email: tapchisinhhoc@vjs.ac.vn