H\(_{2}\)O\(_{2}\) production in Lactobacillus strains isolated from the intestinal microbiome of healthy people

Ha Thi Thu, Hoang The Hung, Tran Xuan Thach, Nguyen Thi Hoa, La Thi Lan Anh, Vu Thi Hien, Nguyen Dinh Duy, Dong Van Quyen, Nguyen Thi Tuyet Nhung

Abstract


Lactobacillus sp. in the digestive tract are capable of producing H2O2 to inhibit the growth of harmful bacteria and balance the gut microflora. In this study, we have isolated 115 strains of Lactobacillus spp. from stool samples of healthy people in Ha Noi. Of the 50 tested Lactobacillus strains, 9 strains were capable of producing H2O2, of which the Lac.VFE-14 strain produced highly H2O2 with a concentration of 2.183 mM, followed by Lac.VFE-08 strains (2.081 mM) and Lac.VFE-04 (2.067 mM). All three strains grew well in MRS medium supplemented with bile salts or adjusted to low pH value. With 0.3% of bile salt, the survival rates of these 3 strains were 99%, 95% and 97%, respectively. At pH 3.0, after 3 hours of cultivation, the survival rates of the three strains were 98.54%, 94.15% and 95.27%, respectively. In addition, each of the cell-free culture supernatants of these three strains that inhibit the growth of S. aureus ATCC-23235. The inhibition zone diameters of the three strains were 19.0±1.0 mm, 14.0±1.0 mm and 11.7±1.3 mm, respectively. The results of 16S rRNA gene analyses showed that Lac.VFE-14, Lac.VFE-08 and Lac.VFE-04 had high similarity scores with L. plantarum ZZU 23 (100%), L. rhamnosus JCM 1136 (99%) and L. plantarum S7 (98.65%), respectively. This study indicates that all three strains have the potential to be used as probiotics in the future.

 

 


Keywords


Lactobacillus, Staphylococcus aureus, H2O2, bile salt, antimicrobial activity.

Full Text:

PDF

References


Belicová A., Mikulášová M., Dušinský R., 2013. Probiotic potential and safety properties of Lactobacillus plantarum from Slovak Bryndza cheese. BioMed research international, 2013(ID 760298): 8 pp.

Bermudez-Brito M., Plaza-Díaz J., Muñoz-Quezada S., Gómez-Llorente C., Gil A., 2012. "Probiotic mechanisms of action." Annals of Nutrition and Metabolism, 61(2): 160–174.

Boateng, M., S. Price, K. Huddersman and S. E. Walsh ., 2011. Antimicrobial activities of hydrogen peroxide and its activation by a novel heterogeneous Fenton’s‐like modified PAN catalyst. Journal of applied microbiology, 111(6): 1533–1543.

Chen C., Lai C., Huang L., Huang Y., Toh S., Weng C., Chuang C., Lu C., H.-J. Tang ., 2019. Antimicrobial activity of Lactobacillus species against carbapenem-resistant Enterobacteriaceae. Frontiers in microbiology, 10: 789.

Corry J.E.L., Curtis G.D.W., Baird R. M., 2003. Handbook of Culture Media for Food Microbiology. Elsevier Science, 37.

Davoodabadi A., Dallal M. M. S., Foroushani A. R., Douraghi M., Harati F. A., 2015. Antibacterial activity of Lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria. Anaerobe, 34: 53–58.

Derrien M., van Hylckama Vlieg J. E., 2015. Fate, activity, and impact of ingested bacteria within the human gut microbiota. Trends in microbiology, 23(6): 354–366.

Thi Bich Thuy Do, Thi Diem Huong Nguyen, 2018. Determination of salt intolerance and probiotic properties of lactic acid bacteria isolated from gut of Decapterus lajang. HUAF journal of agricultural science & technology, Hue University - University of Agriculture and Forestry, 2(2): 799–806 [in Vietnamese].

Eggers S., Barker A. K., Valentine S., Hess T., Duster M., Safdar N., 2018. Effect of Lactobacillus rhamnosus HN001 on carriage of Staphylococcus aureus: results of the impact of probiotics for reducing infections in veterans (IMPROVE) study. BMC infectious diseases, 18(1): 129.

Enitan A., Adeyemo J., Ogunbanwo S., 2011. Influence of growth conditions and nutritional requirements on the production of hydrogen peroxide by lactic acid bacteria. African journal of microbiology research, 5(15): 2059–2066.

Fornitano A., Amêndola I., Santos S., 2019. Lactobacillus rhamnosus versus Staphylococcus aureus: influence on growth and expression of virulence factors. J Dent Maxillofacial Res, 2(2): 29–33.

Hertzberger R., Arents J., Dekker H. L., Pridmore R. D., Gysler C., Kleerebezem M., de Mattos M. J. T., 2014. H2O2 production in species of the Lactobacillus acidophilus group: a central role for a novel NADH-dependent flavin reductase. Appl. Environ. Microbiol., 80(7): 2229–2239.

Johansson M. A., Björkander S., Mata Forsberg M., Qazi K. R., Salvany Celades M., Bittmann J., Eberl M., Sverremark-Ekström E., 2016. Probiotic lactobacilli modulate Staphylococcus aureus-induced activation of conventional and unconventional T cells and NK cells. Frontiers in immunology, 7: 273.

Kang D.-K., Oh H., Ham J.-S., Kim J., Yoon C., Ahn Y., Kim H., 2005. Identification and characterization of hydrogen peroxide-generating Lactobacillus fermentum CS12-1. Asian-australasian journal of animal sciences, 18(1): 90–95.

Nanda., Chaudhary., Kumar., 2018. Molecular Approaches for Identification of Lactobacilli from Traditional Dairy. Advances in Animal Biotechnology and its Applications, 181–196.

Van Thanh Nguyen, Thu Hoa Tran, Vu Tuong Vy Nguyen, 2007. Investigation of acid, bile salt and antibiotic tolerance of some oral probiotics. Pharmaceutical journal, 378: 255–263 [in Vietnamese].

Markowiak P., Śliżewska K., 2017. Effects of probiotics, prebiotics, and synbiotics on human health. Nutrients, 9(9): 1021.

Martín R., Suárez J. E., 2010. Biosynthesis and degradation of H2O2 by vaginal lactobacilli. Appl. Environ. Microbiol., 76(2): 400–405.

Misaghi A., Parsaeimehr M., Akhondzadeh A., Gandomi H., Azizkhani M., 2017. The inhibitory effects of Lactobacillus fermentum, Lactobacillus acidophilus and Lactobacillus paracasei isolated from yoghurt on the growth and enterotoxin A gene expression of S. aureus. Iranian Journal of Veterinary Medicine, 11(2): 191–201.

Tomas Vetrovsky, Petr Baldrian ., 2013. The Variability of The 16S rRNA Gene in Bacterial Genomes and Its Consequences for Bacterial Community Analyses. Plos One 8: (2).

Duc Tinh Quach, Thanh Trung Tong, Ngoc Duy Nguyen, Thuy Huong Nguyen, 2013. Investigation of some probiotic properties of traditional and Lactobacillus casei VTCC186-supplemented Kefir. Science & Technology Development, 16(3): 40–47 [in Vietnamese].

Ren D., Gong S., Shu J., Zhu J., Rong F., Zhang Z., Wang D., Gao L., Qu T., Liu H., 2017. Mixed Lactobacillus plantarum strains inhibit Staphylococcus aureus induced inflammation and ameliorate

intestinal microflora in mice. BioMed research international., 2017(ID 7476467): 7 pages.

Shehata M., El Sohaimy S., El-Sahn M. A., Youssef M.,2016. Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Annals of Agricultural Sciences, 61(1): 65–75.

Urdaneta V., Casadesús J., 2017. Interactions between bacteria and bile salts in the gastrointestinal and hepatobiliary tracts. Frontiers in medicine, 4: 163.

Xianyu Y., Zhu K., Chen W., Wang X., Zhao H., Sun J., Wang Z., Jiang X., 2013. Enzymatic assay for Cu (II) with horseradish peroxidase and its application in colorimetric logic gate. Analytical chemistry, 85(15): 7029–7032.

Zalán Z., Németh E., Baráth Á., Halász A., 2005. Influence of growth medium on hydrogen peroxide and bacteriocin production of Lactobacillus strains. Food Technology and Biotechnology, 43(3): 219–225.




DOI: https://doi.org/10.15625/0866-7160/v42n1.14508 Display counter: Abstract : 146 views. PDF : 45 views.

 

          mitra usaha tani       budidayatani.com

Editorial Office:

1st Floor, A16 Building, 18B Hoang Quoc Viet Street, Cau Giay District, Hanoi, Vietnam

Tel: (+84) 24 3791 7101

Email: tapchisinhhoc@vjs.ac.vn