Study on several biological characteristics affected on the biofilm formation capacity of the phenol-degrading yeast Trichosporon asahii B1 isolated from Ha Long, Quang Ninh

Le Thi Nhi Cong, Cung Thi Ngoc Mai, Nghiem Ngoc Minh


Several phenol-degrading and biofilm-forming yeast strains have been isolated from oil polluted sediment and water samples collected in Halong Bay, Quang Ninh. Amongst, QN-B1 strain with circle, convex, rough and white colony and 3-4 mm of diameter is considered as the best organism to degrade phenol and form biofilm. The yeast strains was then identified by ITS1-5.8rRNA-ITS2 sequencing analysis and showed homological coefficient of 99% with Trichosporon asahii. Based on morphological, bio-physical and bio-chemical characteristics, the strain was named as Trichosporon asahii QN-B1. It was signed in genbank as number KC139404. The influences of initial pH, NaCl concentrations and temperature on biofilm formation were investigated. As a result, the optimal biofilm formation conditions were pH from 3 to 7, NaCl of 1.5% and at 30oC. It also could grow in the minimum mineral salt medium supplemented with 200ppm phenol. Using optimal condition of biofilm formation, the QN-B1 could degrade up to 90% of phenol with the initial concentration of 200 ppm. These results showed potential application of biofilm forming yeasts in treatment of phenol and other aromatic compounds polluted in water in Vietnam.


Trichosporon asahii, biofilm, phenol decomposition, yeast.

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