The study on in vitro culture of embryogenic callus and somatic embryo tissue of Panax vietnamensis Ha et Grushv.

Mai Truong, Tran Thi Ngoc Ha, Phan Tuong Loc, Le Tan Duc, Tran Trong Tuan, Do Dang Giap, Bui Dinh Thach, Pham Duc Tri, Nguyen Duc Minh Hung, Nguyen Thi Thanh, Nguyen Van Ket, Tran Cong Luan, Nguyen Huu Ho

Abstract



This paper presented the results on induction and multiplication of embryogenic calli in liquid medium; on induction of somatic embryo, shoot/root morphogenesis of somatic embryo through culture and on multiplication of somatic embryo tissue in liquid medium. The aim of this study is to lay out the basis for large scale multiplication of these two kinds of tissues with high capable of secondary metabolite production due to their more or less morphological differentiation status. Leaf disks (about 0.5 × 0.5 cm) were cultured on the MS medium with 2 mg/l 2,4-D for callus induction. Callus was subcultured on the MS medium with 1 mg/l 2,4-D + 1 mg/l NAA + 0.2 mg/l kinetin + 10% coconut water for  induction of somatic embryo tissue.  The embryogenic callus was proliferated in the MS½ liquid medium with 0.5 mg/l 2,4-D + 0.5 mg/l NAA. Depending on the initial medium and the subsequent media, the somatic embryo tissue was cultured for development, via two directions, into population of shoots or roots. The mentioned above tissues are being cultured on shaker in big flask/bioreactor for biomass propagation.

Keywords


Panax vietnamensis, cell suspension, embryogenic callus, somatic embryo tissue.

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DOI: https://doi.org/10.15625/0866-7160/v35n3se.3855 Display counter: Abstract : 144 views. PDF : 1768 views.

 

                 

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