A study on root cultures of Hemigraphis glaucescens C. B. Clarke for betuline production

Quach Ngo Diem Phuong, Vu Thi Bach Phuong, Giong Hoi Khoanh, Tran Ngoc Trung, Bui Van Le

Abstract


Up to now, there has not been any  scientific publication related to chemistry and pharmaceutical value of Hemigraphis glaucescens C. B. Clarke. However, they have been used as a popular and effective therapy for hypertensive patients  like Pseuderanthemum bracteatum, which  has been posted in many publications to include medicinal properties of betuline. This research demonstrated that Hemigraphis glaucescens also contains betuline, the same substance in Pseuderanthemum bracteatum. Besides, it focused on screening and finding out antioxidant extract fraction of Hermigraphis glaucescens; accordingly, we can apply in vitro culture technique on getting antioxidant materials actively. In details, by using Ferric cyanine reducing antioxidant power (Yen and Duh) and free radicals scavenging (DPPH) assay, we elucidated that ethanol fraction of root Hermigraphis glaucescens was the best. Adventitious roots were formed by using 0.5 mg/l NAA and hairy roots were also formed by co-culturing with Agrobacterium rhizogenes ATCC 15834 after 10 days.


Keywords


Agrobacterium rhizogenes, Hemigraphis glaucescens, betuline, hairy root culture.

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DOI: https://doi.org/10.15625/0866-7160/v36n1se.4386 Display counter: Abstract : 120 views. PDF : 276 views.

 

                 

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